In Vitro Evidence for Oral Soft Tissue Regenerative Effects of a Polynucleotide-Hyaluronan Formulation.

ABSTRACT:

Objectives

We aimed to investigate the effects of a polynucleotide-hyaluronan (PN-HA) formulation on human palatal fibroblasts (HPFs) and oral epithelial cells (OKF6/TERT-2) to assess its potential application in oral soft tissue reconstructive procedures.

Material and methods

The impact of PN-HA on cell viability, migration, and proliferation was assessed using functional assays. Gene expression of wound healing-related growth factors, inflammatory cytokines, and keratinization markers was evaluated by qRT-PCR, while protein expression and kinase signaling were analyzed via immunofluorescence and immunoblotting.

Results

PN-HA preserved cell viability and significantly (p < 0.05) enhanced migration of both HPFs and OKF6/TERT-2 compared to untreated control cells. Whil

SUBMITTER: Mladenova I 

PROVIDER: S-EPMC12626280 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature