Single-Cell Transcriptomics Uncover EEF1A1-Driven Ubiquitination Dysregulation in T Cell Exhaustion and SLE Pathogenesis via STAT1-Mediated Th1/Th2 Imbalance.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is a complex autoimmune disorder marked by immune dysregulation and multiorgan involvement. This study investigates the role of the ubiquitination-related gene EEF1A1 in SLE pathogenesis, focusing on T cell dysfunction.

Methods

Single-cell RNA sequencing (scRNA-seq) data from the GSE135779 dataset was analyzed to characterize the cellular composition of SLE samples. Clustering analysis identified 15 T cell subpopulations, with seven clusters notably depleted in SLE. Trajectory analysis indicated progressive transcriptional dysregulation during T cell differentiation. High-dimensional weighted gene coexpression network analysis (hdWGCNA) and LASSO regression highlighted EEF1A1 as a key ubiquitination-related hub gene. EEF1A1 expre

SUBMITTER: Xing L 

PROVIDER: S-EPMC12626703 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature