Methods for Analyzing Alternative Splicing and Its Regulation in Plants: From Gene-Specific Approaches to Transcriptome-Wide Studies.

ABSTRACT: Precursor messenger RNA (pre-mRNA) splicing is a fundamental mechanism of gene regulation that influences both mRNA abundance and proteome diversity. In plants, alternative splicing plays a critical role in coordinating development and enabling responses to environmental stress. This process is tightly regulated by the spliceosome and associated splicing factors, which recognize conserved sequence motifs in pre-mRNAs to guide intron removal and exon joining. In this review, we summarize and compare experimental approaches used to analyze both the regulation of alternative splicing and the splicing profiles of genes, spanning from gene-specific assays to transcriptome-wide methods. Gene-specific techniques, such as minigene assays, transient expression systems, electrophoretic mobility shif

SUBMITTER: Vraggalas S 

PROVIDER: S-EPMC12626760 | biostudies-literature | 2025 Nov-Dec

REPOSITORIES: biostudies-literature