Transcriptomic analysis reveals TME-mediated macrophage IFIT1 upregulation and CX3CR1 suppression drive osteosarcoma progression.

ABSTRACT:

Introduction

Osteosarcoma (OS) is one of the most common bone tumors with an unsatisfactory prognosis for patients. Due to the stagnation in conventional treatments, researchers are exploring therapeutic targets from the tumor microenvironment (TME) and tumor-associated macrophages (TAM). Our study investigates how OS TME influences macrophage gene expression, potentially informing OS treatment strategies.

Methods

RNA sequencing was performed on bone marrow-derived macrophages (BMMs) cultured with or without K7M2 conditional medium (CM) for 48 h to analyze gene expression changes. Single-cell sequencing and PCR were used to examine the expression levels of IFIT1 and CX3CR1. Their functions were verified through flow cytometry, cloning, wound healing, and transwell assays usi

SUBMITTER: Wang K 

PROVIDER: S-EPMC12626788 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature