Association of TNF-α polymorphisms rs1800629 (-308G>A) and rs361525 (-238G>A) with type 2 diabetes mellitus in the Punjabi population of Pakistan.

ABSTRACT:

Background

The TNF-α -promoter polymorphisms rs1800629 (-308G>A) and rs361525 (-238G>A) have been variably associated with type 2 diabetes mellitus (T2DM) risk in different populations. This study evaluated these two polymorphisms in a cohort of Punjabi individuals from Pakistan.

Methods

A case-control study was conducted including 100 clinically diagnosed T2DM patients and 100 healthy controls. Genotyping of TNF-α rs1800629 and rs361525 was performed using allele-specific ARMS-PCR and validated by sequencing. Allele and genotype frequencies were compared between groups, odds ratios (ORs) and 95% confidence intervals (CIs) were calculated, and Hardy-Weinberg equilibrium was assessed.

Results

The A alleles of rs1800629 and rs361525 were significantly more

SUBMITTER: Waqar M 

PROVIDER: S-EPMC12626861 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature