A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas.

ABSTRACT: Chloroplast synthetic biology holds promise for advancing photosynthetic organisms through improving the function of plastids. However, chloroplast engineering efforts face limitations due to the scarcity of genetic tools and the low throughput of plant-based systems. To address these challenges, we here established Chlamydomonas reinhardtii as a prototyping chassis for chloroplast synthetic biology. To that end, we developed an automation workflow that enables the generation, handling and analysis of thousands of transplastomic strains in parallel. Furthermore, we expanded the repertoire of effective selection markers and reporter genes, and we characterized over 140 regulatory parts, including native and synthetic promoters, 5' and 3' untranslated regions, and intercistronic expression e

SUBMITTER: Inckemann RM 

PROVIDER: S-EPMC12626891 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature