Identification of disease causing loci using an array-based genotyping approach on pooled DNA.

ABSTRACT:

Background

Pooling genomic DNA samples within clinical classes of disease followed by genotyping on whole-genome SNP microarrays, allows for rapid and inexpensive genome-wide association studies. Key to the success of these studies is the accuracy of the allelic frequency calculations, the ability to identify false-positives arising from assay variability and the ability to better resolve association signals through analysis of neighbouring SNPs.

Results

We report the accuracy of allelic frequency measurements on pooled genomic DNA samples by comparing these measurements to the known allelic frequencies as determined by individual genotyping. We describe modifications to the calculation of k-correction factors from relative allele signal (RAS) values that remove biases and r

SUBMITTER: Craig DW 

PROVIDER: S-EPMC1262713 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature