Pharmacological inhibition of RE1 silencing transcription factor disrupts SOX2 expression and neurogenesis in human induced pluripotent stem cells derived neuronal models.

ABSTRACT:

Background

The role of RE1-Silencing Transcription Factor (REST) in human neurogenesis remains unexplored. This study aims to investigate the expression patterns and possible targets of REST during the early stages of human neurogenesis. Methods: Human neural stem cells and neurons from human induced pluripotent stem cells (hiPSCs) were generated through established protocols. hiPSCs and derived neurons were characterized by immunocytochemistry (SOX2, OCT4, TUJ-1) and qPCR for pluripotency and neuronal marker expression. REST expression and the effect of REST inhibition using X5050 at different stages of development were evaluated using immunostaining. Further, proteomic analysis was performed to identify the key molecules and signalling pathways impacted by the inhibition of REST

SUBMITTER: Nassar A 

PROVIDER: S-EPMC12627181 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature