Molecular characterization of Salmonella isolates from poultry in Egypt: Detection of virulence, ESBLs genes and antimicrobial resistance profiling.

ABSTRACT: This study aimed to determine the prevalence, serovars, virulence gene profiles, antimicrobial resistance patterns, and extended-spectrum β-lactamase (ESBL) genes in Salmonella isolated from broiler chickens in Egypt. In this study, 200 samples were obtained from broiler chickens. Samples were processed using standard bacteriological techniques, and suspected isolates were biochemically identified and molecularly confirmed by PCR targeting the invA gene. Serotyping was performed according to the Kauffmann-White scheme. Virulence genes (hilA, stn, spvC, and spiC) and ESBL genes (bla_TEM, bla_SHV, and bla_CTX-M) were detected by PCR. Antimicrobial susceptibility testing was carried out following CLSI guidelines. Nineteen isolates (9.5%) were confirmed as Salmone

SUBMITTER: Salem M 

PROVIDER: S-EPMC12627184 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature