Hydrogen Sulfide and Methane on Breath Test Correlate with Human Small Intestinal Hydrogen Sulfide Producers and Methanogens.

ABSTRACT:

Background

Evidence indicates that 3 gas breath testing (BT) correlates with stool microbial populations. Breath methane (CH₄) levels correlate with stool Methanobrevibacter smithii levels and constipation, while hydrogen sulfide (H₂S) levels correlate with stool H₂S producers and diarrhea. However, their relationships to small bowel microbes are unknown.

Aims

To assess relationships between small bowel microbes and breath gases.

Methods

REIMAGINE study subjects completed a fasting single-sample BT for CH₄ and H₂S. During esophagogastroduodenoscopy without colon preparation, duodenal aspirates were obtained using double-lumen sterile aspiration catheters. Microbial DNAs underwent shotgun sequencing (NovaSeq6000).<

SUBMITTER: Villanueva-Millan MJ 

PROVIDER: S-EPMC12627187 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature