Construction and application of a humanized mouse model for prostate cancer in immunotherapy.

ABSTRACT: Prostate cancer (PCa) exhibits low sensitivity to immune checkpoint inhibitors due to insufficient T cell infiltration and the dominance of immunosuppressive cells in the tumor immune microenvironment (TIME). Immunotherapy-based combination therapy proves to be an effective strategy in overcoming immune resistance. However, the development and optimization of such therapies necessitate an accurate preclinical model capable of replicating the complex TIME of PCa. To address this need, we developed a humanized mouse model that closely mimics the TIME of PCa patients. This model was created by transplanting human peripheral blood mononuclear cells (PBMCs) into severe combined immunodeficient mice. We systematically investigated factors influencing immune reconstitution, including donor variab

SUBMITTER: Guo W 

PROVIDER: S-EPMC12627316 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature