Detection of Staphylococcus aureus via IgY-based immunomagnetic separation and a phage lysin LysGH15-based colloidal gold immunochromatographic assay.

ABSTRACT:

Introduction

Staphylococcus aureus (S. aureus) is an important human pathogen that frequently causes disease in hospital and community settings. Due to the pervasive emergence of virulent and multidrug-resistant methicillin-resistant S. aureus (MRSA) strains, S. aureus infections have become a leading cause of morbidity and mortality.

Objectives

We aimed to develop a reliable and effective detection method that can be used for preventing and managing infections caused by S. aureus.

Methods

A novel S. aureus detection platform that combines immunomagnetic separation via immunomagnetic beads (IMBs) and the phage lysin LysGH15-based colloidal gold immunochromatographic assay (GICA) (named IMBs-GICA) was developed. IMBs-GICA was performed on 426 consecutive samples, inclu

SUBMITTER: Liu X 

PROVIDER: S-EPMC12627338 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature