Ailanthone induces triple-negative breast cancer cells death involving the inhibition of OTUB1-mediated ERRα deubiquitylation.

ABSTRACT:

Introduction

Triple-negative breast cancer (TNBC) remains the most aggressive subtype of breast cancer, and effective therapeutic strategies are needed. Estrogen-related receptor alpha (ERRα) is considered a promising target for managing TNBC.

Objectives

Here, we aimed to screen natural products to find downregulator of ERRα and elucidate its mechanism of action.

Methods

TNBC cells (MDA-MB-231, MDA-MB-468, MDA-MB-453, and BT-549) were used for in vitro studies, and a subcutaneous MDA-MB-231 tumor model was created for in vivo studies. Immunofluorescence assessed protein distribution, while competitive activity-based protein profiling identified potential target proteins. Co-immunoprecipitation detected protein interactions and modifications, and a luciferase reporter

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12627351 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature