Assessing gut microbial provisioning of essential amino acids to host in a mouse model with reconstituted gut microbiomes.

ABSTRACT: Gut microbial provisioning of essential amino acids (EAAs) represents a critical but poorly understood aspect of mammalian nutrition, with direct implications for host metabolism, growth, and disease resistance. While advances in microbiome research have highlighted the potential significance of these microbial-host nutritional interactions, direct empirical evidence quantifying actual microbial contributions to host EAA supply remains surprisingly limited, particularly under controlled experimental conditions. Here, we show using stable carbon isotope analysis of six EAAs across brain, kidney, liver, and muscle tissues that germ-free mice maintained on a high-protein diet and conventionalized mice with reconstituted gut microbiomes fed a low-protein diet for twenty days exhibit no signifi

SUBMITTER: Ayayee P 

PROVIDER: S-EPMC12627464 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature