SHP1 overexpression ameliorates renal fibrosis via inhibition of STAT3 signaling and glycolysis reprogramming.

ABSTRACT: This study aimed to clarify the role of tyrosine phosphatase SHP-1 on renal fibrosis and its underlying mechanisms. We used a unilateral ureteral obstruction (UUO) rat model to simulate renal fibrosis and achieved overexpression of SHP-1 through lentivirus injection. The effects of overexpression of SHP-1 on extracellular matrix (ECM) deposition, fibrosis degree and key indicators of glycolysis in renal tissue were evaluated. Results showed that overexpression of SHP-1 significantly alleviated renal fibrosis and ECM accumulation induced by UUO. SHP-1 overexpression reduced α-SMA, FN, collagen I, III, HK, PKM2, Bax expression, and lactate levels, while increasing glucose uptake and Bcl-2 expression. Bioinformatics analysis has shown that the transcription factors FOXO (FoxO3a and FoxO1) are

SUBMITTER: Tian H 

PROVIDER: S-EPMC12627518 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature