MAFB regulates hematopoietic stem cell proliferation and maintenance.

ABSTRACT: MAFB, a transcription factor of the large Maf family, is expressed in both fetal liver (FL) and bone marrow (BM) hematopoietic stem cells (HSCs). However, its stage-specific roles remain elusive. Here, we reveal that MAFB plays distinct roles in FL and BM HSCs. Using Mafb-deficient and Mafb-GFP knock-in mouse models, we demonstrate that Mafb deletion enhances proliferation, cell cycle entry, and myeloid differentiation of FL HSCs, leading to enhanced chimerism rate in transplantation assays. However, Mafb-deficient BM HSCs exhibit impaired long-term reconstitution and progressive exhaustion, supported by serial transplantation and reduced colony-forming capacity. HSCs from Mafb^f/f::Tie2-Cre mouse (Mafb cKO) further revealed a significant decline in long-term HSC (LT-HSC) populat

SUBMITTER: Asano S 

PROVIDER: S-EPMC12627586 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature