Knockdown of PATZ1 alleviates chronic heart failure through the USP22/HIF-1α axis.

ABSTRACT: The pathogenesis of chronic heart failure (CHF) is complex and not fully understood. HIF-1α plays a paradoxical role in CHF, and its stability is regulated by the deubiquitinating enzyme USP22. However, the role of USP22 in CHF and its upstream regulatory mechanisms remain unclear. This study aims to investigate the role and regulatory mechanism of the PATZ1/USP22/HIF-1α axis in CHF pathogenesis. An in vitro CHF model was established using angiotensin II (Ang II)-induced H9c2 cardiomyocytes and an in vivo model was created by abdominal aortic constriction (AAC) in rats. Genetic manipulations were performed using shRNAs or overexpression plasmids. Cell viability and cardiac function were assessed by CCK-8, TUNEL staining, Western blot, immunohistochemistry, and hemodynamic measurements. Pro

SUBMITTER: Luo Y 

PROVIDER: S-EPMC12627675 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature