Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species.

ABSTRACT: Microfluidic technologies offer powerful tools for miniaturized and highly controlled biological experiments, yet their application in plant research remains underexploited. In this study, we present a droplet-based microfluidic platform tailored for the encapsulation and cultivation of plant protoplasts, enabling long-term observation of cell development at nearly single-cell resolution. Protoplasts isolated from leaves of Nicotiana tabacum, Brassica juncea, and Kalanchoe daigremontiana were used to evaluate the platform's suitability across diverse plant species. Our results demonstrate species-dependent responses to microfluidic cultivation, with tobacco protoplasts showing the highest viability. The system permits dynamic tracking of cell fate within individual droplets and supports th

SUBMITTER: Marczakiewicz-Perera P 

PROVIDER: S-EPMC12627680 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature