Synergistic enzyme action boosts phenolic compounds in flaxseed during germination using a two-level factorial design.

ABSTRACT: A two-level factorial design in Design-Expert^® software was applied to statistically model and optimize the effects of key endogenous enzymes on the release of total phenolic content (TPC) and total flavonoid content (TFC) during flaxseed germination. The model demonstrated high predictive accuracy (R² > 0.99) and identified germination day (GD), β-glucosidase (β-GL), peroxidase (POX), and catalase (CAT) as critical variables with significant synergistic interactions influencing TPC and TFC biosynthesis. Under these optimal factors, five-day germinated sprouts showed the highest levels of bioactive compounds, with TPC and TFC increasing by 7.6-fold and 38.27-fold, respectively. Compared to dry seeds, High-performance liquid chromatography (HPLC) analysis confirmed marked increas

SUBMITTER: Barakat AZ 

PROVIDER: S-EPMC12627697 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature