Recombinase polymerase amplification of forensic short tandem repeat loci.

ABSTRACT: Short tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS) in centralized laboratories. However, there is an increasing need for a low cost, portable and rapid STR genotyping method. Multiple methods for miniaturization have been explored, all relying on polymerase chain reaction (PCR) for generating amplicons. PCR requires precise thermal cycling, which complicates the design of the STR genotyping microfluidic device. Recombinase Polymerase Amplification (RPA) is an isothermal DNA amplification method that operates between 37 °C and 42 °C and completes within 40 min. This, along with the robustnes

SUBMITTER: Skevin S 

PROVIDER: S-EPMC12627728 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature