Amino acid residues that are important for Hyal2 function as a receptor for jaagsiekte sheep retrovirus.

ABSTRACT:

Background

Infection by jaagsiekte sheep retrovirus (JSRV) and by enzootic nasal tumor virus (ENTV) depends on cell-surface expression of the virus entry receptor, hyaluronidase 2 (Hyal2). Human Hyal2 binds the envelope (Env) proteins of these viruses and is functional as a receptor, but Hyal2 from mice does not bind Env nor does it mediate entry of either virus. Here we have explored the amino acid determinants that account for the difference in receptor function.

Results

Analysis of human-mouse Hyal2 chimeric proteins showed that amino acid differences responsible for the difference in Hyal2 receptor activity were localized to the central third of Hyal2. Human Hyal2 mutants containing single or double amino acid replacements with the respective mouse amino acids were gener

SUBMITTER: Duh FM 

PROVIDER: S-EPMC1262777 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature