Diphtheria toxin T-domain as a tool for inducing lipid vesicle fusion.

ABSTRACT: Membrane fusion is fundamental for many biological processes, including subcellular compartmentalization, cell growth, and exo- and endocytosis. It also plays a key role in the fabrication of artificial membrane structures, particularly vesicles. However, replicating membrane fusion in vitro under neutral pH conditions without prior modification of lipid vesicles with fusion-promoting biomolecules has been a significant challenge, hampering the development of biomimetic fusion systems and their potential applications. Here, we reveal diphtheria toxin T-domain as a fusogenic tool that renders membrane modifications unnecessary. The T-domain spontaneously interacts with anionic nanosized unilamellar vesicles at neutral pH in a non-disruptive manner. We demonstrate that membrane-associated T-

SUBMITTER: Jasko P 

PROVIDER: S-EPMC12627820 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature