Engineering PVX vectors harboring heterologous VSRs to enhance Recombinant vaccine protein expression in plants.

ABSTRACT: Plant-based systems offer a sustainable platform for production of recombinant proteins, but their utility remains limited by host RNA silencing and low yields. Potato virus X (PVX)-derived vectors have shown promise in transient expression systems; however, their efficiency can be further improved by incorporating viral suppressors of RNA silencing (VSRs). Here, we engineered optimized PVX-based expression vectors harboring heterologous VSRs (P19, P38, and NSs) from distinct plant viruses, systematically evaluating the effects of VSR type, insertion position, and transcriptional orientation. Reversing the VSR cassette orientation relative to the target gene alleviated transcriptional interference, significantly improving both target protein and VSR expression. Among the tested VSRs, NSs c

SUBMITTER: Jung S 

PROVIDER: S-EPMC12627851 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature