Integrative multi-omics analysis of autism spectrum disorder reveals unique microbial macromolecules interactions.

ABSTRACT:

Introduction

Gut microbiota alterations have been implicated in Autism Spectrum Disorder (ASD), yet the mechanisms linking these changes to ASD pathophysiology remain unclear.

Objectives

This study utilized a multi-omics approach to uncover mechanisms linking gut microbiota to ASD by examining microbial diversity, bacterial metaproteins, associated metabolic pathways and host proteome.

Methods

The gut microbiota of 30 children with severe ASD and 30 healthy controls was analyzed. Microbial diversity was assessed using 16S rRNA V3 and V4 sequencing. A novel metaproteomics pipeline identified bacterial proteins, while untargeted metabolomics explored altered metabolic pathways. Finally, multi-omics integration was employed to connect macromolecular changes to neurodevel

SUBMITTER: Osama A 

PROVIDER: S-EPMC12627878 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature