Targeted regulation of sterol biosynthesis genes according to perturbations in ergosterol biosynthesis in fungi.

ABSTRACT:

Introduction

The synthesis and regulation of ergosterol are vital for fungal growth and stress adaptation. While ergosterol-mediated feedback regulation is a recognized mechanism controlling sterol biosynthesis in fungi, prior research suggests the presence of additional regulatory mechanisms. However, the specifics of the alternative regulatory mechanisms have not been systematically investigated.

Objectives

We proposed that a regulatory network is likely to discern disturbances in sterol biosynthesis and trigger responses accordingly. This study aimed to validate the hypothesis and investigate the regulatory mechanisms.

Methods

Quantitative Real-time PCR and HPLC-MS/MS were used to explore and compare the regulation of sterol biosynthesis in different fungi. Key tra

SUBMITTER: Yu P 

PROVIDER: S-EPMC12627898 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature