Unlocking River Biofilm Microbial Diversity: A Comparative Analysis of Sequencing Technologies.

ABSTRACT: Freshwater ecosystems are under increasing pressure from pollution, habitat degradation and climate change, highlighting the need for reliable biomonitoring approaches to assess ecosystem health and identify the causes of biodiversity and ecosystem service loss. Characterisation of freshwater microbiomes has the potential to be an important tool for understanding freshwater ecology, ecosystem health and ecosystem function. High-throughput sequencing technologies, such as Illumina short-read and Pacific Biosciences long-read sequencing, are widely used for microbial community analysis. However, the relative performance of these approaches for monitoring freshwater microbiomes has not been well explored. In this study, we compared the performance of long- and short-read sequencing approaches

SUBMITTER: Anderson MAJ 

PROVIDER: S-EPMC12627904 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature