Performance of Two Custom Probe Kits for In-Solution Enrichment of Ancient Avian DNA.

ABSTRACT: Ancient DNA (aDNA) analysis remains challenging due to low endogenous DNA content of degraded samples. Hybridisation-based in-solution enrichment has emerged as an effective tool for targeting genomic regions, enhancing endogenous DNA yield while minimising overall sequencing effort. Despite their widespread use, the performance of different probe kits in capture efficiency remains insufficiently understood, particularly in nonhuman model organisms. In this study, we examined the performance of two commercially available custom probe systems, the RNA-based myBaits and DNA-based Twist, in enriching endogenous aDNA (0.9%-90.1%) extracted from crow bones collected from the early to late Holocene (100-14,000 years ago). The target regions included a panel of 104 K genome-wide single nucleotide

SUBMITTER: Gwee CY 

PROVIDER: S-EPMC12627907 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature