Quantum sensing of free radicals in macrophages reveals early autophagy-lysosome regulation in an atherosclerosis cell model.

ABSTRACT: Atherosclerosis is the predominant cause of death in industrialized countries and is on track to become the foremost cause of death worldwide. Free radicals play a crucial role in regulating the degradation system in macrophages within plaques which lead to complications in atherosclerosis. They are linked to the decline of autophagy-lysosomes and plaque progression. However, their specific production sites and timing remain unclear. To investigate free radical production in the macrophage autophagy-lysosome system's response to stress, we employed nanodiamond-based quantum sensing. By incubating RAW 264.7 macrophages with nanodiamonds and oxidized low-density lipoprotein, we observed colocalization of nanodiamonds with oxLDL in the autophagy-lysosomal system. Quantum sensing was then appl

SUBMITTER: Fan S 

PROVIDER: S-EPMC12628021 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature