Development of a novel circulating miRNA panel for early detection of esophageal cancer.

ABSTRACT:

Objectives

Esophageal cancer (EC) is a highly aggressive malignancy with a low survival rate. Current detection methods like endoscopy are invasive and expensive, resulting in low compliance rates. This study aimed to develop and validate a novel circulating microRNA (miRNA)-based panel for early EC detection and compare its effectiveness in serum versus plasma samples.

Methods

The study developed a multiplexed miRNA assay to detect eight EC-related miRNAs in both plasma (n=45) and serum (n=148) samples. Expression levels of these miRNAs were compared between EC patients and control subjects. The serum-based panel was further validated in an independent cohort and compared with carcinoembryonic antigen (CEA), a conventional tumor marker.

Results

The multiplexed miRNA

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12628261 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature