Cell-Derived Nanocarriers of Apoptotic Bodies with an Antimicrobial Peptide for Targeting Intracellular <i>S. aureus</i> Infections.

ABSTRACT: Intracellular infections caused by Staphylococcus aureus are challenging due to their ability to evade host defenses, through the development of small colony variants (SCVs) that are resistant to conventional therapies. This study yielded and evaluated size-remodeled apoptotic bodies (ReApoBDs) as cell-derived nanocarriers for the targeted delivery of BotrAMP14, an antimicrobial peptide, to treat S. aureus-infected macrophages. ReApoBDs demonstrated high encapsulation efficiencies (∼70%), biocompatibility, sustained drug release over 12 h, colloidal stability, and improved intracellular delivery. The ReApoBDs-BotrAMP14 nanoformulation reduced intracellular bacterial loads while exhibiting lower cytotoxicity compared to the free peptide. Moreover, in vivo experiments de

SUBMITTER: Nieto-Marin V 

PROVIDER: S-EPMC12628330 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature