Reengineered Anti-CD4 Cys-diabody Variants for ⁸⁹Zr-immunoPET of CD4⁺ T Cells in Immunocompetent Mice.

ABSTRACT:

Purpose

CD4⁺ T cells (T helper and T reg) play an important role in the immune system and are influential in autoimmune diseases (e.g., rheumatoid arthritis, inflammatory bowel disease) and cancer (antitumor immunity). Non-invasive, whole-body anti-CD4 immunoPET can provide dynamic and spatial information (localization, proliferation, and migration) on CD4⁺ T cells. The cys-diabody format enables site-specific radiolabeling and rapid renal clearance, which results in high-contrast images at early time points.

Procedures

In this work, an anti-CD4 cys-diabody based on the hybridoma GK1.5 was reengineered by CDR-grafting (GK1.5 FR cDb) for higher expression in mammalian cell lines. An N-glycosylation motif in the variable light chain domain framework was r

SUBMITTER: Salazar FB 

PROVIDER: S-EPMC12628392 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature