Dentin matrix protein-1 promoted osteogenic differentiation of valvular interstitial cells via MAPK signal pathway during aortic valve calcification.

ABSTRACT: Valvular interstitial cells (VICs) are integral to the progression of calcific aortic valve disease (CAVD). Dentin matrix protein-1 (DMP-1), a member of the Sibling family protein, is implicated in the calcification process. This study aims to investigate the role and mechanisms of DMP-1 in the osteogenic differentiation of VICs. Between April 2018 and December 2018, aortic valve tissues were collected from 14 patients undergoing aortic valve replacement or heart transplantation. DMP-1 expression was quantified in calcified valves versus normal controls. An in vitro model of VICs' osteogenic differentiation was established to study the regulatory mechanism of DMP-1 on valvular calcification using immunoblot, immunohistochemistry, immunofluorescence, etc. The expression of DMP-1 was signifi

SUBMITTER: Chen J 

PROVIDER: S-EPMC12628416 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature