RNF185 promotes esophageal squamous cell carcinoma progression by regulating BAK1 ubiquitination and activating the cGAS-STING-IRF3 pathway.

ABSTRACT:

Objective

RNF185 is a mitochondrial RING-type E3 ubiquitin ligase known to regulate mitochondrial homeostasis, apoptosis, and innate immune signaling through ubiquitination of substrates such as BNIP1, JWA, and cGAS. Although dysregulation of RNF185 has been reported in several cancers, its role in esophageal squamous cell carcinoma (ESCC) remains unclear. The present study aimed to investigate the function of RNF185 in ESCC progression and assess its potential as a therapeutic target.

Methods

We analyzed RNF185 expression in the TCGA-ESCA dataset and ESCC cell lines. RNF185 knockout cell lines were generated using CRISPR/Cas9. Functional assays including CCK-8, colony formation, apoptosis analysis, transmission electron microscopy (TEM), and ELISA were performed. Activation

SUBMITTER: Wang KH 

PROVIDER: S-EPMC12628518 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature