WTAP regulates NCOA4-mediated ferroptosis via a YTHDF2-dependent mechanism in preeclampsia.

ABSTRACT:

Background

Preeclampsia (PE) is a pregnancy-specific hypertensive disorder associated with placental dysfunction and oxidative stress. This study explored whether WTAP regulates ferroptosis in trophoblasts through m⁶A-dependent control of NCOA4 and YTHDF2.

Methods

WTAP expression and global m⁶A levels in PE placentas were examined by qRT-PCR, western blot, and immunohistochemistry, along with histopathological analysis. WTAP, NCOA4, and YTHDF2 expression were manipulated in HTR-8/SVneo trophoblasts using siRNAs or overexpression plasmids. Cell proliferation, migration, cell-cycle distribution, oxidative stress, and ferroptosis markers were evaluated. MeRIP-qPCR and RIP-qPCR were used to assess NCOA4 m⁶A methylation and YTHDF2 binding. A PE mo

SUBMITTER: Li C 

PROVIDER: S-EPMC12628531 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature