Multi-omics analysis unveils the role of cancer-associated fibroblasts in cutaneous squamous cell carcinoma.

ABSTRACT:

Background

Human papillomavirus (HPV) infection is associated with an increased risk of cutaneous squamous cell carcinoma (CSCC). A comprehensive understanding of the cellular heterogeneity of HPV-positive and -negative CSCC is crucial for improving diagnosis and preventing tumor progression.

Methods

We conducted an integrated analysis of single-cell RNA and spatial transcriptomic data from different skin tissue sources to map the cellular landscape of the tumor microenvironment (TME) in both HPV positive and negative CSCC. Results were validated through multiplex immunohistochemistry (mIHC) and in vitro experiments.

Results

We identified 10 major cell types in CSCC and normal skin samples, including epithelial cells, myeloid cells, T cells, fibroblasts, endothelial c

SUBMITTER: Wang X 

PROVIDER: S-EPMC12628552 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature