Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.

ABSTRACT:

Study question

At which arrest stage can spermatocytes be rescued by injection into meiotic oocytes?

Summary answer

In mice, spermatocytes arrested at the diplotene stage, but not at the pachytene stage, can resume meiosis within immature oocytes and support full-term embryonic development.

What is known already

In mice, at least some of the spermatocyte arrest mutations can be overcome by injecting spermatocytes into immature oocytes.

Study design size duration

The study was carried out from October 2019 to April 2025. Adult azoospermic mice (at 4-26 weeks of age) from nine strains carrying spermatocyte arrest mutations were used as spermatocyte donors. Adult B6D2F1 females at 9-12 weeks of age were used as oocyte donors for spermatocyte injection. Adult ICR s

SUBMITTER: Ogonuki N 

PROVIDER: S-EPMC12628785 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature