Enriched Long-Read Sequencing of Co-circulating Viruses in Complex Samples.

ABSTRACT: At present, no single workflow is available for quick and accurate identification and analysis of genomes of various viruses present together in a field or clinical sample, particularly when followed by long-read sequencing. Our work addressed this limitation by combining targeted enrichment with long-read, real-time sequencing. Using a panel of probes targeting 16,069 complete viral genomes, we validated this workflow (termed TELSVirus) on complex sample matrices collected from pigs and compared its performance to traditional methods including real-time reverse transcription polymerase chain reaction and shotgun metagenomics. Using serial dilutions of samples with known viral status, we observed that TELSVirus generated viral reads for dilutions up to 10-9. TELSVirus was able to detect vi

SUBMITTER: Meneguzzi M 

PROVIDER: S-EPMC12628787 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature