The C-terminal domain of T9SS component protein SprA assists <i>Flavobacterium psychrophilum</i> bacteriophage endolysin Ely174 to lyse Gram-negative bacteria.

ABSTRACT: Flavobacterium psychrophilum is the pathogen of bacterial cold-water disease, causing enormous economic losses in aquaculture. Antibiotic therapy to control F. psychrophilum risks the development of drug-resistant strains. The use of phage-derived endolysins as effective substitutes for antibiotics is a current research hotspot. In this study, the endolysin Ely174 of F. psychrophilum bacteriophage was heterologously expressed. The optical density of Triton-pretreated F. psychrophilum at 600 nm was decreased from 0.8 to 0.2 in approximately 6 min using 2.5 µg/mL endolysin Ely174. In addition to its wide pH tolerance range, Ely174 also displayed a broad host spectrum. The presence of Mg²⁺, Ca²⁺ and Na⁺ in the reaction system enhanc

SUBMITTER: Xie S 

PROVIDER: S-EPMC12628812 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature