Adenylate kinase gene polymerase chain reaction-restriction fragment length polymorphism to differentiate Escherichia coli, Escherichia fergusonii, and Citrobacter freundii: a molecular diagnostic study.

ABSTRACT:

Background

Escherichia coli (EC), Escherichia fergusonii (EF), and Citrobacter freundii (CF) are clinically significant Gram-negative bacteria that are difficult to differentiate because of their shared 16S rRNA gene sequences.

Methods

This study presents a novel approach utilizing adenylate kinase (adk) gene polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis for the precise discrimination of EC, EF, and CF. Analysis of adk sequences revealed unique restriction sites for BtsIMutI, BtgI, and AgeI restriction enzymes at nucleotide positions 93 and 96.

Results

Theoretical predictions translated into distinct banding patterns during agarose gel electrophoresis following PCR/restriction digestion. Experimental validation with reference strain

SUBMITTER: Dahal RH 

PROVIDER: S-EPMC12628835 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature