Engineering broad-spectrum phage-resistant <i>Escherichia coli</i> via adaptive and programmable defense strategies.

ABSTRACT: Phage contamination, which impacts product quality and production efficiency, remains a major challenge in industrial fermentation. Although bacteria have evolved various defense systems to combat phage infection, these systems often suffer from narrow host specificity and limited efficacy. In this study, we isolated and characterized a novel lytic Escherichia coli phage, TR2, from a contaminated fermentation substrate. Its strong environmental stability, short latency period, and high lytic activity render it a significant threat to fermentation processes. Genomic sequencing revealed that phage TR2 has a linear, double-stranded DNA genome of 45,171 bp with a G+C content of 44% and 74 coding sequences. On the basis of the physiological characteristics and genomic features of this ph

SUBMITTER: Xu Z 

PROVIDER: S-EPMC12628846 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature