The epidermal growth factor receptor regulates YAP signalling and promotes diabetic retinal fibrosis by modulating the degradation of SAV1.

ABSTRACT:

Purpose

The epidermal growth factor receptor (EGFR) and Yes-associated protein (YAP) signalling pathways are two intrinsic mechanisms that typically control fibrosis. The intracellular molecules that regulate these two pathways remain unclear. Here, we investigated how EGFR and YAP signalling control retinal Müller cell-related diabetic retinal fibrosis in vivo and in vitro.

Methods

Human retinal Müller cells (MIO-M1) were transfected in vitro with a Flag-tagged EGFR expression plasmid or an EGFR-targeting siRNA. Western blotting and coimmunoprecipitation (Co-IP) were used to assess the role of EGFR in mediating the interaction between SAV1 and Herc4 and its degradation. In vivo, mice with STZ-induced diabetes received erlotinib (an EGFR inhibitor) intraperitoneally or an in

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12629034 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature