GD2-CAR-Engineered Microglia Exhibit Antitumor Effects in Organoids and Animal Models of Retinoblastoma.

ABSTRACT:

Purpose

Retinoblastoma (RB) is the most prevalent intraocular malignancy in children, which significantly impacts patients' quality of life. Chimeric antigen receptor (CAR) immune cell therapies opened a door to treating tumors. However, whether microglia, the resident immune cell in the retina, could be engineered to treat retinoblastoma remains unknown. The purpose of this study is to generate human CAR-microglia and to investigate the antitumor effects of CAR-microglia in RB.

Methods

The presence of CAR in microglia cells was verified using a fluorescence microscope, PCR, and flow cytometry analysis. The CAR-microglia generated from induced pluripotent stem cells were identified by immunofluorescence, flow cytometry analysis, and RNA sequencing (RNA-seq). The antitumor ef

SUBMITTER: Xu J 

PROVIDER: S-EPMC12629131 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature