Recombinant Expression and Bioprocess Optimization of Priestia megaterium α-Amylase and Its Impact on Dough Fermentation Efficiency.

ABSTRACT: α-Amylase is a key hydrolytic enzyme in starch degradation, playing a crucial role in industrial bioprocesses such as dough fermentation. However, optimizing α-amylase production remains challenging due to variations in microbial sources, culture conditions, and induction strategies. In this study, Priestia megaterium α-amylase (PmAmy) production was optimized for the first time based on the biomass/IPTG ratio in a controlled bioprocess. The impact of enzyme supplementation with equal quantity and enzymatic activity on dough fermentation was also evaluated to ensure consistent performance and effective application. Under the bioprocess conditions of 1.0 vvm airflow, 37°C, and 1000 rpm, a biomass-to-IPTG ratio was optimized as 20 g_biomass mmol_IPTG ^-1 at pH 7

SUBMITTER: Erdem A 

PROVIDER: S-EPMC12629164 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature