Capturing aberrant cell behaviors producing defects in human embryos via live imaging.

ABSTRACT: The generation of human embryos in vitro has revolutionized reproductive medicine, and also made it possible to study fundamental aspects of early human development. However, human preimplantation embryos often display an array of morphological defects associated with poor development and implantation. Here, we used live-embryo imaging and computational analysis to capture how these defects can be produced in real time. We record various forms of mitotic errors including lagging chromosomes producing micronuclei, multipolar spindles causing abnormal chromosome organization recapitulated by daughter cells, and uncontrolled scattering of condensed chromosomes. In addition, we capture abnormal cleavage furrow dynamics during cytokinesis producing binucleated and enucleated cells. Finally, we

SUBMITTER: Akizawa H 

PROVIDER: S-EPMC12629204 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature