Trichinella spiralis serine protease mediates larval invasion of gut epithelium via binding to CK8 and activating RhoA/ROCK1 pathway.

ABSTRACT:

Background

Trichinella spiralis adult worms and larvae parasitize respectively in small intestine and skeletal muscles of the same host, and larval invasion of gut mucosa is the pivotal step for T. spiralis infection. A T. spiralis serine proteinase (TsSPc) was identified in its intestinal infective larva (IIL) ES antigens. TsSPc is involved in larval invasion of gut epithelium, but the mechanism is not completely elucidated. The purpose of this study was to investigate the mechanism of TsSPc action in larval invasion of gut mucosa.

Methodology/principal finding

The results of molecular docking, immunofluorescence assay (IFA), GST pull-down and co-immunoprecipitation (Co-IP) showed that rTsSPc specifically bound to cytokeratin 8 (CK8) receptor in Caco-2 cells and activated R

SUBMITTER: Zheng WW 

PROVIDER: S-EPMC12629419 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature