Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.

ABSTRACT: Tomato (Solanum lycopersicum), a model for fleshy fruit ripening, is predicted to possess ~40,000 genes based on in silico homology-based annotation. However, the functional roles of most annotated genes remain unvalidated. Transposon-tagged mutagenesis offers a powerful strategy for functional genomics, enabling gene identification through phenotypic analysis and activation tagging. Yet, the lack of an efficient in planta transformation system has limited large-scale transposon mutagenesis in tomato. To overcome this limitation, we developed two tomato starter lines, each harboring a maize transposon element: the Dissociation (Ds) element and its corresponding Activator (Ac) transposase. Crossing these lines induced Ac-mediated transposition of Ds in the F1 generation. In the F2 progeny,

SUBMITTER: Kumari A 

PROVIDER: S-EPMC12629433 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature