Functional characterization of a bovine luteal cell culture model: Effects of passage number.

ABSTRACT: Primary cell culture models are useful tools to analyze intracellular signaling pathways and interactions in detail. However, at higher passages, vital cell characteristics such as cell morphology, physiology, gene expression and cell proliferation can be compromised which may result in variable data. In the present study, we characterized cultured primary luteal cells (PLCs) and compared them to intermediate passaged luteal cells i.e. passage number 15 (P15) and high passaged luteal cells i.e. passage number 30 (P30). To explore in-vitro culture induced variabilities, PLCs were passaged repeatedly until passage number P30. Expression of cell cytoskeleton proteins was monitored by immunofluorescence and steroidogenic acute regulatory (STAR) protein expression was detected by capillary elec

SUBMITTER: Sharma A 

PROVIDER: S-EPMC12629482 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature