Combining genome and tissue engineering for next-generation human biomimetics.

ABSTRACT: Tissue engineering for cardiovascular implants has largely utilized primary human cells to generate human tissue-engineered matrices (hTEMs). However, due to donor-to-donor variability and limited passage numbers, a more robust alternative to primary cells would be beneficial. To overcome these limitations, we have defined a new differentiation protocol for human-induced pluripotent stem cells (hiPSCs) into isogeneic cardiac fibroblast-like cells (iCFs) using animal sera-free and chemically defined methods. Morphology, extracellular matrix (ECM) deposition, and global transcriptomics revealed similarity between iCFs and primary human cardiac fibroblasts. Additionally, by overexpressing specific ECM and ECM-related proteins through gene-editing approaches, the ECM composition can be modulat

SUBMITTER: Gahwiler EKN 

PROVIDER: S-EPMC12629539 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature