Fibrinogen triggers perivascular fibroblast activation in a mouse model of cortical ischemic stroke.

ABSTRACT: Fibrotic scar formation caused by stromal cells is often associated with chronic, non-healing pathology impeding repair of the central nervous system (CNS). Perivascular fibroblasts (PVFs) in the perivascular space are activated, express and deposit excess collagen I (Col I), and form a fibrotic scar following CNS disease. Here we show that blood-derived fibrinogen deposition in the perivascular space following photothrombosis, a mouse model for ischemic stroke, initially induces PVF activation. Pharmacological fibrinogen depletion reduces PVF activation and migration away from blood vessels to build up the fibrotic scar. Fibrinogen-induced beta1 integrin signaling in PVF regulates Col I expression. Single-cell RNA sequencing and genetic approches revealed a contribution of fibrinogen-indu

SUBMITTER: Martinez Santamaria JC 

PROVIDER: S-EPMC12629924 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature