Integrative 16S rRNA and transcriptome analysis reveals the molecular mechanisms underlying salt- tolerant germination in highland barley (<i>Hordeum vulgare</i> var. <i>coeleste Linnaeus</i>) seeds.

ABSTRACT: Salt stress is a critical abiotic factor that impairs crop seed germination and limits agricultural productivity. Elucidating the mechanisms governing salt tolerance is essential for development of salt-tolerant crop varieties. In this investigation, 217 accessions of highland barley (Hordeum vulgare var. coeleste Linnaeus) were evaluated. Germination assays conducted under 200 mmol/L and 500 mmol/L NaCl conditions identified a salt-tolerant variety 37 and a salt-sensitive variety 44. By integrating transcriptome sequencing, 16S rRNA sequencing, and Na⁺/K⁺ content analysis, we systematically investigated the molecular mechanisms underlying salt-tolerant germination in highland barley seeds. Our findings revealed that the salt-tolerant variety 37 maintain

SUBMITTER: Ren P 

PROVIDER: S-EPMC12629937 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature